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By Saul Neidleman

"Advances in utilized Microbiology" covers a huge diversity of subject matters within the fields of utilized microbiology and biotechnology. those volumes offer articles which can be of curiosity to biotechnology researchers in academia and undefined, fermentation microbiologists, microbial ecologists, biochemical engineers and utilized microbiologists in different speciality components.

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Latex FIG. 5. , 1991). 43 RELEASE OF GENETICALLY ENGINEERED MICROORGANISMS 21em00 4 c* FIG. 6. Modified perfusion unit for studying transformations of fixed nitrogen in soil (J. D. Doyle and G. Stotzky, unpublished). 44 G. STOTZKY ET AL. , 1991; Kunc and Stotzky, 1980; Macura and Stotzky, 1980; see Stotzky, 1986). If significant changes in nitrogen transformations are observed as the result of inoculating a GEM, the kinetics of nitrogen transformations in subsamples of soil from the master jars should be compared to the kinetics observed in the perfusion technique, to verify the effects of the GEM.

2°C. 03 ml of glacial acetic acid and a small amount (-10 mg) of Bray's nitrate-nitrite powder, containing MnSO, and Zn dust, to each well of the plate containing ammonium-oxidizer broth. The formation of a red color in the wells indicates the oxidation of NH,' to NO,- or NO,-. Determine the numbers of nitrite oxidizers by adding similar amounts of glacial acetic acid and Bray's powder, without MnSO, and Zn dust, to each well of nitrite-oxidizer broth. The absence of a red color is presumptive that NO,- has been oxidized to NO3-.

Zero the spectrophotometer with the controls for the samples and with the 0-pg p-nitrophenol standard solution for the standards. If the absorbance of a sample exceeds that of the standards, dilute the sample with distilled water. Include this dilution when calculation the concentration of p-nitrophenol. Convert the data to an oven-dry basis by multiplying the p-nitrophenol value by 1 plus the percent soil water content (expressed as a decimal). Record the data as micrograms p-nitrophenol per gram soil, oven-dry equivalent.

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