By Kevan M. A. Gartland, Michael R. Davey
Agrobacterium Protocols bargains starting and skilled researchers the main complete choice of step by step protocols for the genetic manipulation of crops utilizing Agrobacterium. the themes variety from the upkeep of bacterial tradition collections to facets of the metabolism and body structure of remodeled tissues and transgenic crops. Drawing at the paintings of top scientists from laboratories all over the world, Agrobacterium Protocols offers a wealth of options for introducing particular DNA sequences into aim plant species and discusses the environmental implications of genetically engineered crops. Its special tactics will facilitate quick move of complex options to different laboratories and their exploitation in basic and utilized plant biology.
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Extra resources for Agrobacterium Protocols (Methods in Molecular Biology Vol 44)
K$g pGA643 H$HpKCBg S I nos-npt * pGA580 St B P -7-7 35s nos - npt E ! KSE* 7 HXSWCBg I L SC 1 cos pGA748 St B P L 1 1 P SC cat- 6b’ w -6a’ Fig. 2. Linear maps of T-DNA region. &I; MI; X, XbaI; Xh, XhoI. E*, not present in pGA748. there is a sequence similar to the T-DNA border, resulting in a partial transfer of the T-DNA. Since, in most binary vectors, a selectable marker is present immediately next to the right border and a foreign DNA is Binary Ti Plasmid Vectors 53 inserted between the selectable marker and left border, transformants often do not carry the foreign DNA.
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