Download Agrobacterium Protocols (Methods in Molecular Biology Vol by Kevan M. A. Gartland, Michael R. Davey PDF

By Kevan M. A. Gartland, Michael R. Davey

Agrobacterium Protocols bargains starting and skilled researchers the main complete choice of step by step protocols for the genetic manipulation of crops utilizing Agrobacterium. the themes variety from the upkeep of bacterial tradition collections to facets of the metabolism and body structure of remodeled tissues and transgenic crops. Drawing at the paintings of top scientists from laboratories all over the world, Agrobacterium Protocols offers a wealth of options for introducing particular DNA sequences into aim plant species and discusses the environmental implications of genetically engineered crops. Its special tactics will facilitate quick move of complex options to different laboratories and their exploitation in basic and utilized plant biology.

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K$g pGA643 H$HpKCBg S I nos-npt * pGA580 St B P -7-7 35s nos - npt E ! KSE* 7 HXSWCBg I L SC 1 cos pGA748 St B P L 1 1 P SC cat- 6b’ w -6a’ Fig. 2. Linear maps of T-DNA region. &I; MI; X, XbaI; Xh, XhoI. E*, not present in pGA748. there is a sequence similar to the T-DNA border, resulting in a partial transfer of the T-DNA. Since, in most binary vectors, a selectable marker is present immediately next to the right border and a foreign DNA is Binary Ti Plasmid Vectors 53 inserted between the selectable marker and left border, transformants often do not carry the foreign DNA.

Chnstlanson, M. , Quatrano, R. S. (1989) A dwarf mutant of Arabidopsis generated by T-DNA insertion mutagenesis. Science 243,1351-1354 18. , Van Montagu, M , and Schell, J (1983) Expresslon of chimeric genes transferred into plant cells using a Ti-plasmld-derived vector. Nature 303,209-213. 19. Ow, D. , Wood, K. , de Wet, J. , Helinski, D. , and Howell, S. H. (1986) Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science 234,856-859 20. Jefferson, R.

2,423-418 6. Palmer, A. C. V. and Shaw, C. H (1992) The role of virA and G phosphorylatlon m chemotaxts towards acetosyrmgone by Agrobacterium tumefaciens J. Gen. Microbial 138,2509-2514 7. Kerr, A. (1974) Sot1 microbiological studies on Agrobacterium radtobacter and biological control of crown gall. Soil Sci 118, 168-172. 8. , Ashby, A. M , Loake, G J , and Watson, M D (1988) One small step* the missing link m crown gall. Oxford Surv. Plant Mol. Cell Biol. 5,177-183 9. Shaw, C. H. (1991) Swlmmmg against the tide chemotaxts m Agrobactertum BioEssays 13,25-29.

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